Cell Lines and Cell Cultures
Eurofins Discoverx Ready-to-Assay V1B Vasopressin Receptor Frozen Cells, 2 Vials of Cells. (HTS136RTA)
The Ready-to-Assay V1B Vasopressin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. vasopressin) based activation of AVPR1B GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses AVPR1B alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of AVPR1B, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen CaS Calcium Sensor Receptor Stable Cell Line, 2 vials. (HTS137C)
The ChemiScreen CaS Calcium Sensor Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. extracellular calcium) based activation of CASR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CASR alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CASR, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay TRH Thyrotropin-Releasing Hormone Receptor Frozen Cells, 2 Vials of Cells. (HTS126RTA)
The Ready-to-Assay TRH Thyrotropin-Releasing Hormone Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. TRH) based activation of TRHR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses TRHR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of TRHR, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
ATCC Primary Renal Cortical Epithelial Cells; Normal, Human
Normal Human Primary Renal Cortical Epithelial Cells are cuboidal with a characteristic pattern of swirled cells isolated from the kidney of a donor. The cells have applications in research related to hypertension, diabetes, oncology, renal fibrosis, inflammation, autoimmune disease, drug screening/development, and toxicology.
Eurofins Discoverx ChemiScreen 5-HT2C Serotonin Receptor Stable Cell Line, 2 vials. (HTS132C)
The ChemiScreen 5-HT2C Serotonin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin/5-HT) based activation of HTR2C GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses HTR2C alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of HTR2C, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay IP1 Prostanoid Receptor Frozen Cells, 2 Vials of Cells. (HTS131RTA)
The Ready-to-Assay IP1 Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin I2) based activation of PTGIR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGIR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PTGIR, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiBrite CXCR3 Chemokine Receptor Stable Cell Line, 2 vials. (HTS003L)
The ChemiBrite CXCR3 Chemokine Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of CXCR3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CXCR3 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of CXCR3, the resulting calcium activity can be assayed in either detection mode. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen mGlu2 Metabotropic Glutamate Receptor Stable Cell Line, 2 vials. (HTS146C)
The ChemiScreen mGlu2 Metabotropic Glutamate Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. glutamate) based activation of GRM2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GRM2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of GRM2, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay α2B Adrenergic Receptor Frozen Cells, 2 Vials of Cells. (HTS157LRTA)
The Ready-to-Assay α2B Adrenergic Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA2B GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADRA2B alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of ADRA2B, the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen MC1 Melanocortin Receptor Membrane Preparation, 1 vial. (HTS156M)
The ChemiScreen MC1 Melanocortin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of MC1R surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
ATCC NCI-H2286; Lung Carcinoma; Human (Homo sapiens)
NCI-H2286 [H2286] is a cell line exhibiting rounded morphology that was isolated from the lungs of a 57-year-old, White female patient with stage 1 small cell lung cancer: adenocarcinoma. This product has applications in cancer research.
